After replacing a Slime tube in the rear tire of my Bianchi Axis twice in 7 days, I thought I would be okay with a new inner tube and Mr. Tuffy liner. During the day, I heard a loud hissing noise behind me which was the presta valve failing and releasing all the air in the tube. Fortunately, I had a spare tube, and I was able to replace the tube, pump it up with the frame pump, and make it home from work. I hope my run of bad luck is over now.